Agent Darkbootie is a prominent member of The Swollen Eyeball Network, but also secretly works as a janitor at NASAPlace. Darkbootie is known as the only Swollen Eyeball member to genuinely take Dib seriously, especially in alien matters. His only major appearance was in Battle of the Planets, where he also becomes the only Swollen Eyeball member other than Dib to appear in person.

Darkbootie worked on repairing an old spaceship meant for monkeys while at his job at NASAPlace. He gave it to Dib in order to pilot the planet Mercury and prevent Zim from taking over Earth while piloting planet Mars. This is his last major role and appearance in the series.

Agent Darkbootie was voiced by John de Lancie, who played the all-powerful Q in Star Trek: The Next Generation.